Measurement of IL-10 serum levels in balb/c mice treated with beta-1, 3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii

Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with sulfadiazine. IL-10 levels were determined by ELISA; the highest levels were found in G2, G3 and G4, and the lowest in G1 (p<0.001). Groups G1 to G5 and G7 had substantially higher levels than G6 (p<0.001). In this study, the highest IL-10 levels were found in groups treated with glucan

Observacoes relativas a acao do itraconazol sobre o Toxoplasma gondii / Observations relating to the itraconazole activity against Toxoplasma gondii

Com o intuito de melhor conhecer o espectro de acao terapeutica do itraconazol, um novo composto triazolico, foi analisada sua eventual acao sobre o Toxoplasma gondii, atraves de observacoes "in vitro" e "in vivo", na infeccao experimental de camundongos. O estudo teve tambem interesse em aprimorar o tratamento da toxoplasmose quanto a inocuidade e a facilidade de execucao. O presente experimento nao mostrou atividade "in vivo" ou "in vitro" do itraconazol na infeccao experimental pelo Toxoplasma gondii em camundongos diversamente do observado em pesquisa previa, em relacao ao Trypanosoma cruzi.